Product Name:

Human GJbeta1 ELISA Kit

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Catalog: E016429
species: Human
Gene: GJbeta1
Specificity: This assay has high sensitivity and excellent specificity for detection of Human GJbeta1. No significant cross-reactivity or interference between Human GJbeta1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human GJbeta1 and all the analogues, therefore, cross reaction may still exist.
Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity: 0.188ng/mL
Intended Use: Human GJbeta1 ELISA Kit allows for the in vitro quantitative determination of GJbeta1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
Storage: For 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specifical Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GJbeta1 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GJbeta1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human GJbeta1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GJbeta1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human GJbeta1. You can calculate the concentration of Human GJbeta1 in the samples by comparing the OD of the samples to the standard curve.