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Alternative name: Tumor necrosis factor receptor superfamily member 11A ELISA KIT; Osteoclast differentiation factor receptor ELISA KIT; ODFR ELISA KIT; Receptor activator of NF-KB ELISA KIT; CD_antigen: CD265Tnfrsf11a ELISA KIT; Rank ELISA KIT; ODFR ELISA KIT
Catalog: E039938
species: Human
Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity: <0.094ng/ml
Intended Use: Human TNFRSF11A/RANK ELISA Kit allows for the in vitro quantitative determination of TNFRSF11A/RANK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
Storage: For 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specifical Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human TNFRSF11A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human TNFRSF11A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TNFRSF11A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human TNFRSF11A can be calculated.